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KMID : 0545120090190121679
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Journal of Microbiology and Biotechnology
2009 Volume.19 No. 12 p.1679 ~ p.1687
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Genetic and phenotypic diversity of parathion-degrading bacteria isolated from rice paddy soils
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Choi Min-Kyeong
Kim Kyung-Duk
Ahn Kyong-Mok
Shin Dong-Hyun
Hwang Jae-Hong
Ka Jong-Ok
Seong Chi-Nam
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Abstract
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Three parathion-degrading bacteria and eight pairs of bacteria showing syntrophic metabolism of parathion were isolated from rice field soils, and their genetic and phenotypic characteristics were investigated. The three isolates and eight syntrophic pairs were able to utilize parathion as a sole source of carbon and energy, producing p-nitrophenol as the intermediate metabolite during the complete degradation of parathion. Analysis of 16S rRNA gene sequence indicated that the isolates were related to members of the genera, Burkholderia, Arthrobacter, Pseudomonas, Variovorax, and Ensifer. The chromosomal DNA patterns of the isolates obtained by polymerase-chain-reaction (PCR) amplification of repetitive extragenic palindromic (REP) sequences were distinct from each other. Ten of the isolates had plasmids. All of the isolates and syntrophic pairs were able to degrade parathion-related compounds such as EPN, p-nitrophenol, fenitrothion, and methyl-parathion. When analyzed with PCR amplification and dot-blotting hybridization using various primers targeted for the organophosphorus pesticide hydrolase genes of previously-reported isolates, most of the isolates did not show positive signals, suggesting that their parathion hydrolase genes had no significant sequence homology with those of the previously-reported organophophate pesticide-degrading isolates.
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KEYWORD
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parathion-degrading bacteria, parathion, diversity, organophosphorus insecticide, syntrophic metabolism
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